FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13338519
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC140050.057.924026120
GAGCACA223950.038.30834120
AGAGCAC278750.029.939753118-119
AAGAGCA455250.019.471666120
GAAGAGC491700.018.410679120
GGAAGAG579000.015.539569118-119
CGGAAGA638150.014.368492120
TCGGAAG645150.014.046743118-119
GATCGGA763050.012.832418120
ATCGGAA763200.012.447548120
AGATCGG859100.011.318458118-119
TAGATCG242100.011.115726120
CTAGATC67650.010.256533118-119
ACACGTC47200.08.792706120
GAGATCG316800.08.5868118-119
AAGATCG293350.08.499528118-119
TTAGATC94100.08.476177118-119
CAGATCG298300.08.195447120
CACACGT45050.07.197137120
ATAGATC117500.07.0089087118-119
Produced by FastQC (version 0.11.5)