FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12740060
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC139550.066.68759120
GAGCACA216250.043.460484120
AGAGCAC273100.034.232903118-119
AAGAGCA434200.021.62992118-119
GAAGAGC466000.020.097403116-117
GGAAGAG583100.016.62543120
TCGGAAG678200.014.662648120
CGGAAGA675750.014.257933118-119
GATCGGA781600.012.663248118-119
ATCGGAA794600.012.431965120
AGATCGG856450.011.557207120
CTAGATC60450.011.423822120
TAGATCG248400.011.12029120
GAGATCG280300.09.539595116-117
TTAGATC103800.09.504129120
AAGATCG326900.09.244885116-117
CAGATCG235300.08.972253120
ACTAGAT23200.08.212409118-119
TCTAGAT31200.08.107085116-117
CCCTAGA7002.3682333E-67.5123463106-107
Produced by FastQC (version 0.11.5)