FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11684985
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC142300.073.006744120
GAGCACA219700.046.738598120
AGAGCAC283500.037.893986120
AAGAGCA436550.024.261803118-119
GAAGAGC482750.021.632132118-119
GGAAGAG593000.017.84325116-117
TCGGAAG710900.014.978606114-115
CGGAAGA709200.014.949383118-119
GATCGGA834300.012.92417112-113
ATCGGAA832800.012.866843114-115
CACGTCT45650.012.100173120
AGATCGG921500.011.655172116-117
TAGATCG219500.011.324273120
CACACGT41350.010.9506445118-119
CTAGATC47400.010.597988114-115
ACACGTC50350.09.906606118-119
ACGTCTG53750.09.881447120
GAGATCG412050.09.331717110-111
CAGATCG326100.08.797991114-115
TTAGATC72450.08.789141116-117
Produced by FastQC (version 0.11.5)