FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13183695
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC203500.099.69663120
GAGCACA324600.057.87807120
AGAGCAC439650.044.586155118-119
AAGAGCA651250.029.678188118-119
GAAGAGC728000.026.843254116-117
GGAAGAG885900.021.712635116-117
CGGAAGA1057700.018.514193114-115
TCGGAAG1088500.017.627028114-115
ATCGGAA1265850.015.591297112-113
GATCGGA1292450.014.926976112-113
AGATCGG1404400.014.0221110-111
CACGTCT56300.013.617419120
CACACGT54950.013.070793120
TAGATCG328150.012.543303110-111
CTAGATC88300.012.476307108-109
TTAGATC126050.011.973272108-109
GAGATCG438650.011.793806110-111
CCGATCT62950.011.73264
ACACGTC56700.011.528732118-119
AAGATCG504350.011.440791116-117
Produced by FastQC (version 0.11.5)