FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13637691
Sequences flagged as poor quality0
Sequence length50-126
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC197100.097.52775120
GAGCACA333600.057.552696120
AGAGCAC451300.042.91886120
AAGAGCA672650.029.357403120
GAAGAGC765900.025.654549118-119
GGAAGAG931850.021.254833118-119
CGGAAGA1089950.018.090704116-117
TCGGAAG1135000.017.416655116-117
ATCGGAA1326300.014.956682114-115
GATCGGA1374850.014.487193114-115
CACGTCT50000.013.267689120
AGATCGG1501650.013.238398112-113
TAGATCG356300.012.304034112-113
CTAGATC67650.011.530269110-111
ACACGTC52300.011.356797120
GAGATCG658100.010.952637112-113
CAGATCG602350.010.524774112-113
CACACGT49100.010.327216118-119
ACGTCTG65750.010.206817120
TTAGATC102000.09.266435112-113
Produced by FastQC (version 0.11.5)