FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14138775
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135500.066.59679120
GAGCACA215000.043.434525120
AGAGCAC287500.032.00382118-119
AAGAGCA455500.020.494936118-119
GAAGAGC522100.018.338135120
GGAAGAG619050.016.249548120
TCGGAAG715600.014.102921120
CGGAAGA724650.013.917748120
ATCGGAA845950.012.189743118-119
GATCGGA856450.012.043739120
TAGATCG230900.011.0687685120
AGATCGG944200.011.014672120
CTAGATC47000.010.109104118-119
CACACGT45800.09.872844120
CACGTCT49550.09.654679120
TTAGATC79500.08.98568114-115
GAGATCG430550.08.462718120
AAGATCG325050.08.104655120
CCTAGAT20150.07.8053937120
CAGATCG352250.07.6093016118-119
Produced by FastQC (version 0.11.5)