FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11570070
Sequences flagged as poor quality0
Sequence length69-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG59600.012.682844120
AAAAAAA808750.012.165276120
CGTATCA373500.09.701495120
CGCCGTA447550.09.409964120
GTCGCCG521200.09.334882120
GTGGTCG634000.09.311041120
GGTCGCC548750.09.270725120
GCCGTAT442650.08.890697120
GTATCAT374200.08.865715120
ATCATTA332350.08.754643120
TGGTCGC581950.08.721216120
TCGCCGT498800.08.575421120
TCATTAA290600.08.546674120
GGTGGTC692800.08.2869835120
CCGTATC427050.08.232112120
TCTCGGT897300.07.728809120
CGGTGGT761050.07.713297118-119
TCGGTGG804450.07.688684120
CTCGGTG841750.07.6045527120
TATCATT412250.07.479883120
Produced by FastQC (version 0.11.5)