FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11570070
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152050.085.85456120
GAGCACA239500.050.713215120
AGAGCAC322050.039.13707118-119
AAGAGCA491150.025.907637120
GAAGAGC548850.023.317467120
GGAAGAG680200.018.718458118-119
CGGAAGA797200.016.329102120
TCGGAAG811800.015.810368118-119
ATCGGAA944350.013.781268118-119
GATCGGA959800.013.468143116-117
CACACGT45850.012.462277120
AGATCGG1046850.012.439184116-117
TAGATCG217300.011.866567120
CTAGATC57500.010.956896118-119
AAGATCG502600.010.618761116-117
TTAGATC99500.010.380975120
GAGATCG303450.010.3082120
CACGTCT48450.010.281518120
ACACGTC48100.010.051734120
TCTAGAT30000.09.767443120
Produced by FastQC (version 0.11.5)