FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12675686
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC159900.069.89371120
GAGCACA248300.043.64531120
AGAGCAC320900.035.228344120
AAGAGCA474100.023.787523120
GAAGAGC545450.020.792772118-119
GGAAGAG649900.018.12463120
CGGAAGA759600.015.193974118-119
TCGGAAG774950.014.906133118-119
ATCGGAA906950.012.898029120
GATCGGA940750.012.470638120
AGATCGG1038750.011.489846120
TAGATCG230600.010.491667114-115
CTAGATC65900.09.66737120
AAGATCG461250.09.242302120
TTAGATC85250.09.062585118-119
GAGATCG354700.08.38774118-119
GGACCCT13000.07.820345106-107
TAAGATC163950.07.605855118-119
CAGATCG363250.07.4631166120
CACACGT51850.07.4506125120
Produced by FastQC (version 0.11.5)