FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19776753
Sequences flagged as poor quality0
Sequence length62-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG111700.09.455297120
TCGGTGG701850.08.5672035120
TGGTCGC505850.08.363404120
GTGGTCG553600.08.357453120
GGTGGTC599750.08.294686120
CGCCGTA435050.08.248551120
GGTCGCC494650.08.213085120
GATCTCG915600.08.120471120
ATCTCGG851200.08.051007120
AGATCTC950250.07.86857120
CTCGGTG747550.07.7785597120
TAGATCT1016250.07.6587005120
TCTCGGT783150.07.6548424120
GCCGTAT415750.07.6355286120
CGGTGGT666650.07.633323120
CGTATCA333050.07.441428120
CCGTATC385050.07.246887120
GTGTAGA1266450.07.192823120
GTAGATC1242700.07.0695286120
GAGTGTA1371500.06.96567120
Produced by FastQC (version 0.11.5)