FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19776753
Sequences flagged as poor quality0
Sequence length50-126
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC211300.080.07881120
GAGCACA342300.049.788857120
AGAGCAC472950.037.09606120
AAGAGCA745200.023.952984120
GAAGAGC806450.022.175798120
GGAAGAG977300.018.604391120
CGGAAGA1106750.016.300879118-119
TCGGAAG1168050.015.549779118-119
ATCGGAA1367700.014.042646120
GATCGGA1437200.013.033259120
AGATCGG1583800.011.98114118-119
TAGATCG479000.011.1708975120
CACACGT55700.010.227527120
CACGTCT60400.09.768522120
AAGATCG606300.09.74736116-117
GAGATCG462350.09.419964116-117
CTAGATC140650.09.125935118-119
TTAGATC230450.08.640541112-113
CAGATCG437950.08.629773114-115
TAAGATC258350.08.114468118-119
Produced by FastQC (version 0.11.5)