FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14899461
Sequences flagged as poor quality0
Sequence length73-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG13950.018.316294120
GTCTAGG10500.014.346417114-115
TCTAGGG11950.013.412185116-117
GTGGTCG257100.09.377627120
AGTCTAG14709.094947E-119.359426120
CTAGGTG10750.09.341854114-115
TAGATCT443900.08.796469120
ATCTCGG405300.08.696668120
TGGTCGC235250.08.5498120
GAGAGTC42300.08.518626120
AGATCTC431000.08.482116120
TCTAGGT15457.2759576E-128.481032120
CGAGTAG28500.08.368743114-115
GATCTAG14650.08.354463112-113
GTAGATC561300.08.3222685120
CTCGGTG358100.08.30613120
GTGTAGA576650.08.282519120
AGTGTAG593750.08.2425995120
GATCTCG438450.08.21845120
ATCTAGG14150.08.2061112-113
Produced by FastQC (version 0.11.5)