FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14899461
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC155600.069.66081120
GAGCACA245700.044.7841120
AGAGCAC322500.033.997017120
AAGAGCA510050.022.191502120
GAAGAGC562950.020.701319120
GGAAGAG683700.017.36228120
TCGGAAG822250.014.987982120
CGGAAGA821100.014.482069118-119
ATCGGAA967050.013.62008120
GATCGGA987050.012.931256118-119
AGATCGG1113550.011.533237120
TAGATCG251950.011.055177120
CTAGATC67000.010.232717116-117
CACACGT42600.09.933419118-119
CACGTCT55900.09.753949120
TTAGATC100500.09.171909116-117
AAGATCG439750.08.981302118-119
ACACGTC58250.08.683784120
GAGATCG388550.08.46784118-119
CAGATCG395400.08.321157116-117
Produced by FastQC (version 0.11.5)