FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14293123
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC220150.0108.535995120
GAGCACA360950.064.84031120
AGAGCAC510400.046.557026118-119
AAGAGCA761750.030.704914118-119
GAAGAGC848600.028.068047116-117
GGAAGAG1002100.023.194965116-117
CGGAAGA1171800.020.282526114-115
TCGGAAG1215050.019.059193114-115
ATCGGAA1409550.016.873774112-113
ACACGTC52600.016.509716120
GATCGGA1437750.016.163855112-113
AGATCGG1561100.015.202492110-111
CACGTCT57000.014.933594120
CACACGT48250.014.137232118-119
TAGATCG387650.013.822478110-111
ACGTCTG66100.013.137837120
GAGATCG534750.012.3226385110-111
CAGATCG512300.012.301576110-111
CTAGATC104300.012.09282118-119
TTAGATC148950.011.981636110-111
Produced by FastQC (version 0.11.5)