FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14963827
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135850.066.35736120
GAGCACA208400.045.99656120
AGAGCAC285850.034.07266120
AAGAGCA457400.021.93881120
GAAGAGC515800.019.219158118-119
GGAAGAG665200.015.365122120
TCGGAAG753000.013.4798155120
CGGAAGA798650.012.789661120
GATCGGA879450.011.826175120
ATCGGAA884400.011.593124120
AGATCGG960500.011.322563120
TAGATCG237250.010.743285118-119
CTAGATC62600.08.962722118-119
AAGATCG430550.08.389909120
TTAGATC102950.08.252693118-119
GAGATCG345450.08.092888114-115
CAGATCG319250.07.150317118-119
CCTAGAT26550.06.764949108-109
TAAGATC180600.06.6434903120
ATAGATC119250.06.3449736118-119
Produced by FastQC (version 0.11.5)