FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15358118
Sequences flagged as poor quality0
Sequence length50-126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC147000.038.503654120
GAGCACA185500.031.477278120
AGAGCAC236550.026.641136120
AAGAGCA369350.017.54693120
GAAGAGC416000.015.475366120
TCGGAAG545350.013.955254120
GGAAGAG518200.013.554852120
CGGAAGA616700.012.000385120
ATCGGAA696600.011.899878120
GATCGGA686350.011.8797455120
AGATCGG769350.011.223898120
TAGATCG159750.011.127602120
CTAGATC46400.010.375919120
TTAGATC63750.09.488462120
GCTAGAT22050.08.397743120
AAGATCG377250.08.327957120
CCTAGAT17200.07.894856120
CACACGT43750.07.7595153118-119
TCTAGAT22400.07.4397110-111
CACGTCT48500.06.9995627120
Produced by FastQC (version 0.11.5)