FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17113767
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC178950.085.31354120
GAGCACA288500.051.468613120
AGAGCAC378900.040.667393120
AAGAGCA593000.026.197493120
GAAGAGC664450.024.603458120
GGAAGAG842450.019.64853120
CGGAAGA1006600.016.66384120
TCGGAAG1026950.016.21034118-119
ATCGGAA1207400.013.722612120
GATCGGA1252950.013.425242120
AGATCGG1380050.012.217389120
TAGATCG348200.011.873293120
CTAGATC88250.011.533037120
AAGATCG533500.09.716271120
TTAGATC133100.09.662249120
GAGATCG524150.09.317594120
CAGATCG432850.08.547594114-115
CACACGT45450.08.506107120
ATAGATC181850.07.7228107120
CACGTCT46800.07.5863934120
Produced by FastQC (version 0.11.5)