FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16634255
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC156400.062.39935120
GAGCACA232350.040.538807120
AGAGCAC314750.032.433365120
AAGAGCA505500.020.080425120
GAAGAGC568800.018.20671120
GGAAGAG685850.015.810484120
TCGGAAG784700.013.9987120
CGGAAGA816450.013.422885120
ATCGGAA934450.012.304658120
GATCGGA931050.012.058686118-119
AGATCGG1038500.011.133608120
TAGATCG206050.09.93095114-115
AAGATCG512150.08.995283120
CTAGATC58850.08.706248110-111
TTAGATC98550.08.463974120
CACACGT47150.08.301102120
GAGATCG361350.07.901682120
TAAGATC205500.07.7745476120
CAGATCG365350.07.5693007120
CACGTCT55700.06.9116807120
Produced by FastQC (version 0.11.5)