FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11821844
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC132200.069.81839120
GAGCACA208800.044.04547120
AGAGCAC281750.034.910385120
AAGAGCA426050.023.742983120
GAAGAGC492200.020.579067120
GGAAGAG577650.018.030594120
TCGGAAG693250.015.76365120
CGGAAGA683900.015.161217120
ATCGGAA810100.013.366569120
GATCGGA831250.013.030164118-119
AGATCGG942000.011.791883120
TAGATCG197850.011.191365118-119
CTAGATC51250.010.329909114-115
TTAGATC78100.09.8908205116-117
AAGATCG393200.08.55295120
GAGATCG377250.08.47325120
CCTAGAT19000.08.411931120
CAGATCG345350.08.233917120
TAAGATC129300.07.416552120
GCTAGAT22650.07.3501663116-117
Produced by FastQC (version 0.11.5)