FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12975605
Sequences flagged as poor quality0
Sequence length67-126
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG12250.015.381356114-115
TCTAGGG11950.015.303683116-117
CGTCTAG13900.012.877738114-115
CGTAGTG33850.010.6574955120
GAGAGTC32200.010.388802120
GTGGTCG253650.09.59379120
CGAGTAG22850.09.070625114-115
GGTCGCC232250.08.952714120
TCGAGTA37850.08.83803120
TCGGTGG331100.08.637309120
GGTGGTC285050.08.629018120
CGTATCA171200.08.50551120
TGGTCGC232950.08.503091118-119
AGATCTC459850.08.387109120
GCGTCGG65950.08.254951120
AGTCTAG18350.08.221346120
GATCTCG439300.08.21207120
TAGATCT480400.08.205832120
ATCTCGG403450.08.177668120
TCTCGGT369700.08.12582120
Produced by FastQC (version 0.11.5)