FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12975605
Sequences flagged as poor quality0
Sequence length50-126
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC143350.068.811195120
GAGCACA221750.043.88502120
AGAGCAC293750.033.85067120
AAGAGCA472550.021.449324120
GAAGAGC495700.020.594711120
GGAAGAG618900.016.42464118-119
CGGAAGA713150.014.696193120
TCGGAAG723350.014.058233120
ACACGTC47200.013.061552120
CACACGT41950.012.641887120
ATCGGAA860000.012.402563120
GATCGGA874950.011.996362118-119
AGATCGG968500.011.389575120
CACGTCT48650.011.173388120
TAGATCG265250.010.621538120
CTAGATC60850.09.58685120
ACGTCTG58400.08.967432120
TTAGATC114800.08.950426120
GAGATCG351750.08.574942120
AAGATCG346650.08.526936114-115
Produced by FastQC (version 0.11.5)