FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13091084
Sequences flagged as poor quality0
Sequence length70-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT239000.08.684942120
AGATCTC245600.08.622538120
GTGTAGA324950.08.400084120
GAGTGTA349300.08.2438755120
AGTGTAG324000.08.221039120
AAGAGTG431300.08.137015120
AGAGTGT383450.07.8069344120
ATCTCGG240200.07.6675057120
GTAGATC326550.07.568961120
TCTCGGT222450.07.551172120
TAGGGAA595200.07.3981285120
GTAGGGA617800.07.2245994120
GATCTCG272000.07.14603120
TGTAGAT364050.07.0200033120
TCGTGTA798250.06.996801120
TCGGTGG209900.06.8308415120
CGTGTAG749600.06.746628120
GTGGTCG165700.06.734094120
GTGTAGG711550.06.7280035120
TGTAGGG661400.06.721137118-119
Produced by FastQC (version 0.11.5)