FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13091084
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC112450.052.230556120
GAGCACA166350.039.56823120
AGAGCAC212750.032.604465120
AAGAGCA345250.019.660854118-119
GAAGAGC370800.019.509342120
GGAAGAG454550.016.388186120
TCGGAAG534550.013.935554120
CGGAAGA545250.013.66797118-119
GATCGGA643050.012.558623120
ATCGGAA647650.012.1860285120
AGATCGG718950.010.990782118-119
TAGATCG166950.010.349332120
CTAGATC41300.09.6544285116-117
AAGATCG309650.08.114383120
TTAGATC72700.07.9221506120
GAGATCG298750.07.584272118-119
CAGATCG297150.06.6346555116-117
CCTAGAT17900.06.187622118-119
TGAGATC125950.06.1807766120
TAAGATC117650.06.078867120
Produced by FastQC (version 0.11.5)