FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14488231
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT99800.014.4884124
TCCGATC106400.014.1535813
CTTCCGA113100.013.529751
AAAAAAA1796850.013.370486120
CGATCTA25050.011.7360685
TTCCGAT164950.09.2388032
CGTATCA739350.09.137788120
TCATTAA596350.09.05107120
GTATCAT713600.08.987407120
TCTCGGG34450.08.549323120
CGCCGTA955800.08.489685120
CCGTATC830050.08.392745120
TGGTCGC1227500.08.3048120
ATCATTA661200.08.263345120
GGTCGCC1160050.08.259171120
GGTGGTC1408900.08.12291120
CATTAAA584850.08.108814120
TATCATT739300.08.081472120
GCCGTAT894450.08.070711120
GTGGTCG1322650.07.984573120
Produced by FastQC (version 0.11.5)