FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14488231
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC217700.099.84022120
GAGCACA356600.060.789757120
AGAGCAC482050.045.06657118-119
AAGAGCA705650.030.687366118-119
GAAGAGC805750.026.930206116-117
GGAAGAG975550.022.158678116-117
CGGAAGA1151850.019.014462114-115
TCGGAAG1173650.018.416424114-115
ATCGGAA1362650.016.126583112-113
GATCGGA1416750.015.316635112-113
CCGATCT109100.014.8718334
TCCGATC116800.014.3498743
AGATCGG1533600.014.323262110-111
CTTCCGA121550.013.9401961
TAGATCG318350.013.191368110-111
CACACGT61600.012.153234120
ACACGTC72700.011.881904120
CTAGATC86200.011.667502110-111
CACGTCT63700.011.494278120
TTAGATC118350.011.448292116-117
Produced by FastQC (version 0.11.5)