FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15203086
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA1213050.014.477129120
GCGTCGG112450.011.097939120
CGTATCA619600.010.448368120
TCATTAA447050.010.267005120
GTATCAT607250.010.26565120
CATTAAA435000.010.247973120
ATCATTA513200.010.147792120
TATCATT613250.09.470573120
CCGTATC713000.09.399443120
GCCGTAT772550.09.389406120
CGCCGTA857900.09.077713120
GTCGCCG1034650.08.617153120
TCGCCGT968800.08.49686120
TGGTCGC1092600.08.473116120
GGTCGCC1054200.08.349213120
GTGGTCG1190850.08.217418120
ATTAAAA496600.07.973989120
TCGGTGG1515600.07.6996937120
GGTGGTC1332000.07.571849120
CGGTGGT1454300.07.376528120
Produced by FastQC (version 0.11.5)