FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15203086
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC229150.074.80311120
GAGCACA346000.049.585377120
AGAGCAC465800.037.940533120
AAGAGCA654450.026.828426118-119
GAAGAGC746700.023.539701118-119
GGAAGAG908300.019.342993116-117
CGGAAGA1016600.017.407202116-117
TCGGAAG1025600.017.02565114-115
CACGTCT59600.014.86487120
ATCGGAA1194650.014.828905114-115
GATCGGA1231450.014.285855112-113
AGATCGG1385050.012.848751112-113
CACACGT54800.012.230612120
TAGATCG230100.011.800896110-111
ACACGTC61450.011.408512120
CTAGATC61350.011.15918110-111
AAGATCG667400.010.632541110-111
TTAGATC84050.010.433355116-117
CGTCTGA93450.09.975061120
ACGTCTG84350.09.666776118-119
Produced by FastQC (version 0.11.5)