FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11264657
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC129850.065.70404120
GAGCACA196950.044.206505120
AGAGCAC258900.033.810482120
AAGAGCA395150.023.224321120
GAAGAGC445200.020.122473118-119
GGAAGAG561950.016.144705118-119
TCGGAAG641200.014.144341116-117
CGGAAGA665100.013.711689116-117
ATCGGAA777050.012.337949120
GATCGGA783050.011.9329405118-119
AGATCGG858750.011.021586118-119
CTAGATC52250.010.587604118-119
TAGATCG196500.010.476465116-117
ACACGTC47000.09.297965120
GACTAGA7404.3137043E-49.085329120
TTAGATC76150.09.026602114-115
AAGATCG369550.08.528939114-115
CACACGT42050.08.47388120
CACGTCT43300.08.3845215120
GCTAGAT19850.08.276459116-117
Produced by FastQC (version 0.11.5)