FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15806357
Sequences flagged as poor quality0
Sequence length62-126
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA207050.011.08394120
AGTGTAG191050.010.974491120
TAGATCT132650.010.96009120
AGAGTGT240250.09.852327120
AAGAGTG274300.09.74631120
GTAGGGA456450.09.160561120
GTGTAGA202550.08.779412120
CGTGTAG587600.08.72114120
TAGGGAA428350.08.698096118-119
AAAGAGT333350.08.398301120
GTGTAGG558000.08.387052120
TGTAGGG530300.08.270034120
GTAGATC226350.08.042075120
CGTCGTG758500.08.031347120
GTCGTGT658400.08.029692120
TCGTGTA619700.08.017335120
AGATCTC149450.07.8789105120
GCGTCGG130350.07.604645120
AGCGTCG852450.07.4985747120
GGGAAAG507900.07.4637513120
Produced by FastQC (version 0.11.5)