FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12668109
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC137350.064.1155120
GAGCACA217050.042.00736120
AGAGCAC286050.034.26044120
AAGAGCA441100.022.3979120
GAAGAGC494950.019.954384118-119
GGAAGAG616550.016.862476120
TCGGAAG696250.014.827536120
CGGAAGA710700.014.288343118-119
ATCGGAA835300.013.279438120
CTAGATC44850.013.001305120
GATCGGA850100.012.557213118-119
TAGATCG166850.012.072969120
AGATCGG929350.011.46501118-119
GCTAGAT21850.09.401064120
TTAGATC63500.08.921956118-119
GAGATCG402200.08.484725116-117
CCTAGAT17750.08.212815118-119
AAGATCG354250.08.164827116-117
CAGATCG388300.07.747386120
TCTAGAT21450.07.5685277116-117
Produced by FastQC (version 0.11.5)