FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15539360
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC185300.069.297455120
GAGCACA276950.045.485226120
AGAGCAC373050.035.74576120
AAGAGCA585600.023.014183120
GAAGAGC653250.020.703388120
GGAAGAG810200.016.951775120
CGGAAGA956600.014.881074120
TCGGAAG950250.014.6641445118-119
ATCGGAA1114000.012.76031118-119
GATCGGA1123250.012.498494120
AGATCGG1235800.011.527239116-117
CTAGATC59100.010.880817120
TAGATCG273700.010.733473120
CACACGT52550.010.176062120
CACGTCT56750.09.780777120
TTAGATC97600.09.432231120
AAGATCG466200.09.180683118-119
GAGATCG458350.08.792398110-111
CAGATCG418050.08.452253112-113
ACACGTC60600.07.971391118-119
Produced by FastQC (version 0.11.5)