FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12730046
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC172700.092.45401120
GAGCACA287200.055.83414120
AGAGCAC388950.041.44366118-119
AAGAGCA586550.027.96397120
GAAGAGC658750.024.388668118-119
GGAAGAG816400.019.92253120
CGGAAGA966800.016.831188120
TCGGAAG974450.016.648003118-119
ATCGGAA1139050.014.296699114-115
GATCGGA1175600.013.874079116-117
ACACGTC50900.012.907808120
CACACGT48550.012.9031118-119
CACGTCT51550.012.8932495120
AGATCGG1279000.012.774708112-113
TAGATCG275600.012.640924114-115
ACGTCTG67450.011.552874120
CTAGATC66700.011.396998114-115
AAGATCG475800.010.397468110-111
TTAGATC97450.010.191249116-117
GCACACG68050.010.103834120
Produced by FastQC (version 0.11.5)