FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12155454
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC134800.073.050865120
GAGCACA213700.049.1246120
AGAGCAC296850.036.49427120
AAGAGCA449650.023.981363118-119
GAAGAGC523400.021.531027120
GGAAGAG635850.017.290737120
CGGAAGA764850.014.856836120
TCGGAAG759650.014.760136118-119
ATCGGAA887800.012.81847116-117
GATCGGA907000.012.513599120
AGATCGG1013900.011.703665120
CACACGT38500.011.150863120
TAGATCG234750.010.37286118-119
CTAGATC60300.010.011851120
CACGTCT41300.09.095512120
TTAGATC91600.09.007451114-115
GAGATCG385150.08.708219120
ACACGTC44200.08.498911118-119
AAGATCG367650.08.484196114-115
ACGTCTG54900.08.430745120
Produced by FastQC (version 0.11.5)