FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13888558
Sequences flagged as poor quality0
Sequence length62-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT165050.010.68646120
AGTGTAG232500.010.68266120
GAGTGTA254900.010.0027895120
GTGTAGA241400.09.891174120
AAGAGTG328650.09.747869120
AGAGTGT285700.09.596393120
TAGGGAA496000.09.482797120
GTAGGGA530650.09.474099120
GTGTAGG639500.08.827772120
TGTAGGG578000.08.812151120
GTAGATC264800.08.790545120
GTCGTGT788000.08.671607120
TCGTGTA730200.08.626796120
CGTGTAG682950.08.274924120
AGGGAAA571250.08.139121120
CGTCGTG899950.08.059535120
ATCTCGG176800.07.940277120
AGATCTC186400.07.9175577120
AGCGTCG1017150.07.879951120
AAAGAGT391000.07.7484827118-119
Produced by FastQC (version 0.11.5)