FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13888558
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC141000.058.679394120
GAGCACA210700.038.311096120
AGAGCAC274900.032.458508120
AAGAGCA425800.021.636238120
GAAGAGC480950.019.52209120
GGAAGAG596950.016.520243120
CGGAAGA714150.014.488511120
TCGGAAG705150.014.476831120
ATCGGAA863200.012.607266120
GATCGGA886000.012.474867120
AGATCGG993200.011.19185120
CTAGATC58800.010.555988116-117
TAGATCG215150.010.2803120
TTAGATC87250.08.6306305116-117
AAGATCG432000.08.292524118-119
GAGATCG422150.07.52322120
GCTAGAT26050.07.1359916116-117
CAGATCG407150.07.0729694120
CACACGT45900.07.0016036120
CACGTCT45850.06.7343664120
Produced by FastQC (version 0.11.5)