FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13285393
Sequences flagged as poor quality0
Sequence length54-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCT284600.010.015711120
GAGTGTA423950.09.342269120
AGAGTGT463150.08.953223120
AGTGTAG381650.08.9154005120
AAGAGTG517350.08.583616120
AGATCTC293400.08.529017120
GTAGATC412550.08.334912120
GTGTAGA392000.08.257557118-119
TGTAGAT415700.08.242883120
TAGGGAA764400.08.062557120
TGTAGGG864900.07.9027977120
TCGGTGG234750.07.8479433120
GTAGGGA807300.07.73818120
ATCTCGG295800.07.4860244120
GGTGGTC202100.07.3936024120
TCTCGGT252200.07.3763185120
CTCGGTG263850.07.3235526120
AGGGAAA809150.07.2013383120
GTGTAGG929700.07.190604120
GATCTCG358800.07.175099120
Produced by FastQC (version 0.11.5)