FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13285393
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC127450.066.860916120
GAGCACA197200.043.310146120
AGAGCAC276300.033.82734120
AAGAGCA425400.021.77409120
GAAGAGC528650.017.751717118-119
GGAAGAG618700.015.742186120
TCGGAAG696000.013.827105120
CGGAAGA721700.013.181558116-117
ATCGGAA831650.012.385595120
GATCGGA864450.012.184083120
AGATCGG950350.010.937687118-119
CTAGATC50650.09.717981112-113
TAGATCG206200.09.361268116-117
CACACGT41800.08.789805120
TTAGATC76400.08.690096120
AAGATCG436600.07.913939118-119
CACGTCT54350.07.5903387120
TCTAGAT24200.07.312231108-109
CCTAGAT18100.07.2874804116-117
TAAGATC152300.07.237305120
Produced by FastQC (version 0.11.5)