FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13550501
Sequences flagged as poor quality0
Sequence length50-126
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAAA792700.010.141917120
CGTATCA333200.09.291136120
GTATCAT337300.08.680157120
TGGTCGC556200.08.640237120
TCATTAA285300.08.369613120
GTGGTCG609300.08.320504120
GGTCGCC558050.08.106294120
GCCGTAT448500.07.932614120
CGCCGTA493300.07.8811207120
GGTGGTC644700.07.845019120
TCGGTGG749700.07.8426466120
CCGTATC405650.07.779619120
ATCATTA332400.07.400259120
CTCGGTG806150.07.3976636120
CGGTGGT719000.07.24294120
TATCATT391750.07.167388120
TCTCGGT832750.07.1289454118-119
GTCGCCG620050.07.0828567120
ATCTCGG898500.06.997893120
TCGCCGT586850.06.9417143120
Produced by FastQC (version 0.11.5)