FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12201476
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG520700.08.918112120
AGATCTC370550.08.786855120
TAGATCT379850.08.777318120
GAGTGTA562050.08.646783120
GATCTCG366100.08.516254120
CTCGGTG305900.08.405147120
GGTGGTC233300.08.394288120
AAGAGTG675050.08.3562355120
GTGTAGA508800.08.335633120
GTGGTCG210500.08.1334305120
TGGTCGC192150.08.066039120
GTAGATC507250.08.053188120
ATCTCGG345000.08.044589120
AGAGTGT607800.07.862443118-119
TGTAGAT531850.07.838831120
GGTCGCC198400.07.6908264120
TAGGGAA947950.07.686991120
TCTCGGT316900.07.5826106120
TCGGTGG278900.07.387995120
TGTAGGG1056250.07.160445120
Produced by FastQC (version 0.11.5)