FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12324743
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC152750.076.72547120
GAGCACA245050.045.704433120
AGAGCAC330800.036.596916120
AAGAGCA505650.023.46953120
GAAGAGC552100.021.578657118-119
GGAAGAG666050.017.697018118-119
CGGAAGA782200.015.379467116-117
TCGGAAG792100.015.008768120
ATCGGAA957500.013.076563120
GATCGGA960100.012.679472118-119
AGATCGG1050050.011.566812112-113
TAGATCG221550.011.385377120
CACGTCT48700.011.109294120
CTAGATC56000.010.790339120
CACACGT43700.010.129402120
TTAGATC89150.09.378866120
AAGATCG443500.09.078547116-117
ACACGTC50950.08.82633118-119
GAGATCG362300.08.533745116-117
CAGATCG373900.08.325371116-117
Produced by FastQC (version 0.11.5)