FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14559435
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC222750.0100.69283120
GAGCACA366050.059.022232120
AGAGCAC491500.044.906055118-119
AAGAGCA706550.031.403133120
GAAGAGC824050.026.915306118-119
GGAAGAG988850.022.471746118-119
CGGAAGA1149900.019.255875116-117
TCGGAAG1182450.018.665956116-117
ATCGGAA1377300.016.18293112-113
GATCGGA1437300.015.48089114-115
AGATCGG1575750.014.261554110-111
TAGATCG340900.013.689889110-111
CACGTCT69000.012.790145120
CTAGATC72450.012.699166108-109
ACGTCTG82150.012.060299120
TTAGATC94650.011.260058108-109
CACACGT62850.011.193608118-119
GAGATCG635500.011.136294112-113
CAGATCG642300.010.759138112-113
ACACGTC75600.010.407076118-119
Produced by FastQC (version 0.11.5)