FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830419

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830419
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14030080
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC199500.087.88449120
GAGCACA319000.051.96212120
AGAGCAC425450.040.2668118-119
AAGAGCA635500.027.324179120
GAAGAGC721900.023.699324116-117
GGAAGAG868100.019.959398118-119
CGGAAGA1000350.017.186749114-115
TCGGAAG1035300.016.935144120
CACGTCT55400.015.063864120
ATCGGAA1208750.014.319318112-113
GATCGGA1247950.014.009946114-115
ACACGTC58000.013.7285595120
CACACGT51850.013.659211118-119
AGATCGG1374500.012.667513110-111
TAGATCG306900.012.149675114-115
CTAGATC64700.011.893675112-113
TTAGATC96450.011.11336120
GAGATCG546000.010.061983112-113
GCTAGAT24600.09.959431120
CAGATCG506450.09.676041112-113
Produced by FastQC (version 0.11.5)