FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13635581
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC163050.079.0231120
GAGCACA283600.046.054424120
AGAGCAC373600.035.958275120
AAGAGCA560350.023.95017118-119
GAAGAGC641200.020.729305118-119
GGAAGAG749300.018.028303120
CGGAAGA864500.015.359213120
TCGGAAG902900.014.856205120
ATCGGAA1039100.013.189557120
GATCGGA1064500.012.728346118-119
AGATCGG1191650.011.540921120
TAGATCG274350.011.259053114-115
CTAGATC70300.011.045717108-109
TTAGATC106150.09.838344120
AAGATCG479500.09.037134116-117
CAGATCG379100.08.640464110-111
GAGATCG435750.08.567189116-117
CACGTCT51100.08.227958120
ATAGATC127300.08.177083114-115
TAAGATC178300.07.796882116-117
Produced by FastQC (version 0.11.5)