FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14359429
Sequences flagged as poor quality0
Sequence length50-126
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC195650.084.942116120
GAGCACA320100.054.046505120
AGAGCAC427350.039.702023118-119
AAGAGCA612950.027.973787118-119
GAAGAGC716950.023.550816116-117
GGAAGAG835850.020.397188116-117
CGGAAGA971350.017.37459114-115
TCGGAAG980600.017.22266114-115
ATCGGAA1140900.014.929485112-113
GATCGGA1185150.014.441348112-113
CACACGT64550.013.224761120
AGATCGG1319900.013.011192110-111
CACGTCT68800.012.180158120
TAGATCG253100.011.788032114-115
CTAGATC58600.011.744302116-117
ACACGTC80850.011.333484120
TTAGATC75200.011.231682114-115
GAGATCG555050.010.130306110-111
CGTCTGA94350.010.126876120
CAGATCG513850.09.717601112-113
Produced by FastQC (version 0.11.5)