FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12617799
Sequences flagged as poor quality0
Sequence length50-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG51450.08.513076120
GATCTCG304400.08.101141120
AGTGTAG420500.07.9761777120
TAGATCT313350.07.8414674118-119
GTGGTCG198550.07.7662663120
AGATCTC316500.07.715601120
CCCCCCC8657.323901E-47.63023041
TGGTCGC184800.07.467495120
TGTAGGG793650.07.42389120
CTCGGTG272400.07.378817120
GTCGCCG190200.07.2870293120
GAGTGTA450700.07.2287183120
ATCTCGG295900.07.1983414120
GTAGATC420900.07.1275477120
AGAGTGT487800.07.1094346120
TCGGTGG260050.07.0601454120
GTAGGGA745900.07.030398120
TAGGGAA724600.06.9638066120
TCTCGGT293100.06.960047120
GTGTAGA424850.06.7788286120
Produced by FastQC (version 0.11.5)