FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12617799
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC131150.057.559135120
GAGCACA218750.033.366673120
AGAGCAC276700.030.871536120
AAGAGCA456300.018.39747120
GAAGAGC505450.016.824284118-119
CGGAAGA610350.015.465397120
TCGGAAG628650.015.178298120
GGAAGAG578900.015.110066120
ATCGGAA741300.012.9454365118-119
GATCGGA743950.012.877593120
AGATCGG838350.011.68744120
TAGATCG226050.010.715799120
TTAGATC76800.010.179721120
GAGATCG326600.09.300353120
CTAGATC59000.09.232319118-119
CAGATCG280750.08.822133118-119
AAGATCG258050.08.4932995116-117
ACACGTC52500.08.056046120
ATAGATC124000.07.596839120
CACACGT52950.07.5034842120
Produced by FastQC (version 0.11.5)