FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12689512
Sequences flagged as poor quality0
Sequence length74-126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG90050.010.478173120
TCGGTGG333550.09.783822120
GTGGTCG250650.09.590957120
TAGATCT483400.09.262309120
AGATCTC468600.09.259863120
CTCGGTG371100.09.247296120
TGGTCGC232550.09.2261715120
ATCTCGG413700.09.108602120
AGTGTAG668750.09.094658120
GGTGGTC284650.09.057654120
GTGTAGA639700.09.000338120
GATCTCG462350.08.852091120
GTAGATC622700.08.802086120
TCTCGGT378150.08.791355118-119
AGAGTGT783850.08.717604120
GGTCGCC229550.08.351856120
GAGTGTA719300.08.330187120
AAGAGTG859400.08.209986120
TGTAGAT659600.08.081884120
CGGTGGT339950.07.6726313120
Produced by FastQC (version 0.11.5)