FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13860700
Sequences flagged as poor quality0
Sequence length50-126
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC136650.067.504524120
GAGCACA217100.042.48961120
AGAGCAC285300.033.625916120
AAGAGCA458150.022.055805120
GAAGAGC509400.019.887632120
GGAAGAG631000.016.115978118-119
TCGGAAG741150.013.633479116-117
CGGAAGA756600.013.564608118-119
ATCGGAA868850.012.054838120
GATCGGA863050.011.948338120
AGATCGG949200.011.75729120
TAGATCG270750.010.703925118-119
GAGATCG376000.09.503398120
TTAGATC102250.09.4963255120
CTAGATC60850.09.356987112-113
CCTAGAT23100.08.12669120
AAGATCG326650.08.125103120
CAGATCG307900.07.595076118-119
AGAGATC154950.07.243382118-119
ATAGATC136950.07.18471120
Produced by FastQC (version 0.11.5)