FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13112586
Sequences flagged as poor quality0
Sequence length50-126
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC135350.061.99358120
GAGCACA226550.038.446777120
AGAGCAC283100.031.105272120
AAGAGCA479150.019.750874120
GAAGAGC500600.018.509136120
GGAAGAG592250.016.442747120
CGGAAGA687250.015.117585120
TCGGAAG693450.014.724581120
GATCGGA818600.012.933689120
ATCGGAA836550.012.439182118-119
AGATCGG925500.011.839968120
ACACGTC36400.011.753928120
TAGATCG242700.010.998061120
CACACGT35750.010.360515118-119
CTAGATC61500.010.175612120
CACGTCT37800.09.46032120
GAGATCG316650.09.408345116-117
TTAGATC92250.09.275729120
AAGATCG335600.09.095683118-119
CAGATCG295450.08.796705120
Produced by FastQC (version 0.11.5)