FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004830441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004830441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12080055
Sequences flagged as poor quality0
Sequence length50-126
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACAC172100.086.36198120
GAGCACA272050.051.90131120
AGAGCAC363100.039.8781118-119
AAGAGCA524250.027.276274118-119
GAAGAGC621200.023.188988120
GGAAGAG749700.019.148458116-117
CGGAAGA880500.016.539053120
TCGGAAG892250.016.026794120
CACGTCT53750.014.803543120
ATCGGAA1026000.014.252132120
GATCGGA1056900.013.671787118-119
AGATCGG1164400.012.593375110-111
CACACGT52850.011.64655118-119
TAGATCG232000.011.598785112-113
CTAGATC62000.011.561735112-113
TTAGATC80300.011.123753116-117
ACACGTC58850.010.586926120
CGTCTGA78900.010.465355120
AAGATCG409600.010.464226110-111
GAGATCG397450.010.331017120
Produced by FastQC (version 0.11.5)